B-Cell Linker anticorps (pTyr84) (Alexa Fluor 647)

N° du produit ABIN1177037

L’anticorps Souris Monoclonal anti-B-Cell Linker a été validé pour ICS. Il convient pour détecter B-Cell Linker dans des échantillons de Humain et Souris. Il y a 2+ publications disponibles.

Aperçu rapide pour B-Cell Linker anticorps (pTyr84) (Alexa Fluor 647) (ABIN1177037)

Antigène: B-Cell Linker (BLNK)

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp B-Cell Linker est conjugé à/à la Alexa Fluor 647

Application: Intracellular Staining (ICS)

Clone: J117-1278

Détail du produit anti-B-Cell Linker anticorps (Alexa Fluor 647)

Épitope: pTyr84

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human BLNK Peptide

Isotype: IgG2b kappa

Information d'application

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Références pour anticorps anti-B-Cell Linker (Alexa Fluor 647) (ABIN1177037)

Pighi, Gu, Dalai, Barbi, Parolini, Bertolaso, Pedron, Parisi, Ren, Cecconi, Chilosi, Menestrina, Zamò: "Phospho-proteomic analysis of mantle cell lymphoma cells suggests a pro-survival role of B-cell receptor signaling." dans: Cellular oncology (Dordrecht), Vol. 34, Issue 2, pp. 141-53, (2011) (PubMed).

Espeli, Mancini, Breton, Poirier, Schiff: "Impaired B-cell development at the pre-BII-cell stage in galectin-1-deficient mice due to inefficient pre-BII/stromal cell interactions." dans: Blood, Vol. 113, Issue 23, pp. 5878-86, (2009) (PubMed).

Détails sur B-Cell Linker

Antigène: B-Cell Linker (BLNK)

Autre désignation: BLNK

Sujet: B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk. Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCg2, leading to the activation of downstream signaling pathways. The J117-1278 monoclonal antibody recognizes the phosphorylated Y84 of BLNK.

Pathways: BCR Signaling